Torrent Suite Software 5.4 Help

Analysis details

The Analysis Details report displays the following information:

Parameter

Description

Run Name

Name of the run.

Run Date

Date and time the Ion PGM™ or Ion Proton™ run was started.

Run Cycles

Number of Ion PGM™ or Ion Proton™ cycles analyzed for this report. Note that this number can differ from the total number of cycles run on the sequencer.

Run Flows

Number of Ion PGM™ or Ion Proton™ nucleotide flows analyzed for this report. Note that this number can differ from the total number of flows occurring on the sequencer.

Project

Names of the projects the result set is a member of.

Sample

Name of the sample assigned to the run used to generate this analysis. This is assigned on theIon PGM™ or Ion Proton™ Sequencer.

Sample Tube Label

The label or written text on a sample tube used to track each sample through the sequencing workflow.

Reference

Name of the library assigned to the run used to generate this analysis. This library name is used to specify the reference genome used for alignment.

Instrument

Name of the sequencing instrument on which the run was performed.

Flow Order

Flow order selected on Ion PGM™ or Ion Proton™ Ion Proton™ Sequencer: Samba = TACGTACGTCTGAGCATCGATCGATGTACAGC [Default]Regular = TACG The "regular" flow order adds bases most rapidly to sequenced molecules but is vulnerable to phase errors. The Samba flow order consists of a 32-base sequence, repeated. This flow order resists phase errors by providing opportunities for out-of-phase molecules to catch up and is designed to sample all dimer (nucleotide pair) sequences, efficiently. Samba is the default flow order because it improve sequencing accuracy for longer reads by resisting phase errors.

Library Key

A short known sequence of bases used to distinguish the library fragment from the test fragment. Example: "TCAG"

TF Key

A short known sequence of bases used to distinguish the test fragment.

Chip ID

The ID number of the chip that appears on the chip barcode label.

Chip Check

A series of tests on reference wells (about 10% of the chip in non-addressable areas) is performed to ensure that the chip is functioning at a basic level. The value of this field is either Passed or Failed.

Chip Type

Type of chip used on the Ion PGM™ Sequencer. Usually, 314, 316, or 318 (for the Ion 314™ chip, Ion 316™ chip, and Ion 318™ chip.) A letter follows the numbers, indicating the chip version.

Chip Data

In this release, the value is single, for a forward run.

Chip Lot Number

The lot number of the chip as scanned by the Ion Proton™ Sequencer or Ion S5™ Sequencer. Not available for Ion PGM™ runs.

Barcode Set

The name of the barcode set assigned to the run. Blank for non-barcode libraries.

Analysis Name

Name of the analysis provided in Torrent Suite™ Software when the analysis was started. If the analysis was scheduled to auto-start, this is the default analysis name.

Analysis Date

Date the analysis was performed.

Analysis Flows

Number of Ion PGM™ orIon Proton™ nucleotide flows analyzed for this report. Note that this number can differ from the total number of flows occurring on the Ion PGM™ orIon Proton™ Sequencer.

runID

The run code that the Torrent Suite™ Software assigned to the planned run for this analysis.

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