The barcode_bias values show the mean signal deviation by flow: how much the observed signal is off from the expected signal. Low bias values, for example with the value shown here, are indications of good signal.

Bias values around 0.33 indicate a signal that is about a third of a base off. Values near 0.5 indicate a signal that is half a base off. Values in this range indicate a problem with the sequencing run or with the barcode classification.

? "barcode_bias": [ 0.026, -0.028, -0.034, 0.011, -0.019, -0.001, 0.072,? -0.061, 0.103, -0.008, -0.062, 0.110, -0.021, 0.001],