Summary of the recommended workflow
Here is an overview of the recommended workflow for a barcode run. Screenshots and more details are provided below.
- Create a template for your runs in the Plan tab Template page. In the template wizard Kits page, select one of the available barcode sets from the drop-down Barcode Sets menu, and fill out the other run information. Save your template.
- When you have the actual sample name, click the Plan Run button for your template. Enter you run name and sample name, then click Plan.
- The Torrent Suite™ Software assigns a name to your planned run, and generates a 5-character code for your planned run name. Your run information is stored in the Torrent Suite™ Software as a planned run until you are ready to start the run on the sequencer.
- When you are ready to start the run, on the Ion S5™ Run Selection screen you select your run from a list of planned runs. Torrent Suite™ Software populates the Ion S5™ Detail screen with the information you entered in the Planning tab. (You may optionally change information on the Run Info screen.)
- You start the Ion S5™ sequencer run as usual.
- When the run and report are complete, you can review the performance of the barcoded reads in the default report page. The following additional barcode-specific files are available for download from the File Links download section: