Use the ERCC_Analysis plugin to determine if a problem exists with either the library preparation or the sequencing instrument run. The ERCC_Analysis plugin determines the relative abundance of the actual versus expected number of ERCC transcript reads for sequencing runs that include ERCC RNA Spike-in Controls.

It assumes that the RNA-Seq or AmpliSeq-RNA library run was spiked with ERCC DNA sequences.

The ERCC_Analysis plugin takes approximately 2–3 minutes to complete for sequencing runs with ≤1,000,000 total reads, and 1–2 minutes longer for each additional million total reads. For example, a run with 5 million total reads may take 10–15 minutes. If the Torrent Suite™ Software is busy performing additional processing functions, plugin run times are longer.