The barcode distance histogram shows, in signal space, the number of reads at various squared residual distances between the predictedsignal and the observed signal.

The distance fields are given in 0.2 increments:

• The first field gives the number of reads with a squared residual distance of between 0 and 0.2.

• The second field gives the number of reads with asquared residual distance of between 0.2 and 0.4.

• The third field gives the number of reads with a squared residual distance of between 0.4 and 0.6, etc.

Smaller distances reflect better matches of the read to barcode. Larger distances reflect poorer matches.

This example reflects the pattern that is typical of a real barcode:

• The most reads have shorted distance residuals.

• Fewer reads have larger distance residuals.

• The entry 5342 in the fifth field tells us that reducing --barcode-cutoff to 0.8 would cause those 5342 reads not to be assigned to a barcode.

? "barcode_distance_hist": [ 907546, 50122, 10793, 4498, 5342 ],