Torrent Suite Software 5.4 Help

Ternary encoding

One side effect of operating in flow space is that barcodes are not limited to binary sequences. For example, each flow can correspond to 0, 1, or 2 bases in a ternary encoding scheme. This scheme allows for a greater number of codewords occupying the same number of flows. However, a flowspace representation must correspond to a legitimate sequence that yields these flow-space values. For example, we cannot have a flow of T, C, T with values 1, 0, 1. For a flow of T, C, T, both T bases are consumed in the first flow, and the sequencing reaction yields the incorrect values 2, 0, ?.