Barcode sets are designed to efficiently separate reads from each other in the presence of errors. Ion Torrent™ sequencing technology produces raw data in flow space. These reads are best described as having a homopolymer run of length 0, 1, 2, etc., ... in flow 1, 2, 3, etc. Because of this characteristic, the most typical error patterns involve either over- or under-estimation of a homopolymer signal in a flow. The most effective barcodes designs for Ion Torrent™ technology are those with distinctive flow-space representations.