View ERCC transcript details

There are two ways to look at the details of a particular ERCC transcript. To view all the details about a particular ERCC transcript, you should view both.

Hover your mouse-cursor over a point in the ERCC Dose Response plot to display a popup window that shows details about that transcript. Overlapping points on the plot can be resolved by zooming in on the plot to more easily distinguish points.

To zoom in on a selected area, click‑drag your mouse to highlight the area of interest.
Double‑click in the plot, or click the Reset Zoom button to zoom out to the full view of the ERCC Dose Response plot.

Parameter	Description
log2 relative ERCC concentration	The log (base 2) of the relative ERCC transcript concentration.
log2 ERCC counts	The log (base 2) of the mapped reads to an ERCC transcript.
transript	The ERCC transcript identifier including length in base pairs (bp).
reads	The number of reads that map to the particular transcript.
mean mapping quality	. Points in the display are color coded based on the mapping quality.

Scroll to the particular transcript, then click the [+] next to the transcript name.

Parameter	Description
Reads	The number of reads that map to the particular transcript.
Coverage Depth	The number of reads covering bases in the transcript.
Coverage	The number of base positions covered by at least one read. Also expressed as a percentage of the full length.
Start Sites	The number of base positions that are the start site for a read.
Unique Start Sites	The number of base positions that have only one read starting at the position.
Coverage CV	Coefficient of Variation for coverage = average coverage / stddev coverage for the entire transcript.