Torrent Suite Software 5.4 Help

Analyze Ion AmpliSeq™ on Ion Chef™ samples

Ion AmpliSeq™ on Ion Chef™ samples can be analyzed.

The process involves creating a Torrent Suite™Sample Set, preparing an Ion Chef™ library, creating a Torrent Suite™ planned run, preparing an Ion Chef™ or OneTouch2 template, and sequencing on an PGM™, Ion Proton™, or Ion S5™ sequencer.

Create Sample Set:

  1. Import samples from a file or enter them manually.
    Note:

  2. This example imports samples from a file.
  3. In the Plan tab, click Samples, then click Import Samples from File.
  4. On the Import Samples window, click Sample File Format button. A CSV template downloads.
  5. Click it, then enter sample names, PCR Plate positions, and DNA or RNA at minimum. Save to your desktop.
  6. Now, click the Select File button, select your CSV file, then click Open.
  7. Click Add Sample Set.
  8. Name your new sample set.
  9. Set Library Prep Type to AmpliSeq on Chef.
  10. Set Library Prep Kit to Ion AmpliSeq Kit on Chef DL8.
  11. Enter PCR plate serial number, then click Save & Finish The software creates your new sample set..
  12. In the Plan tab, click Samples > Sample Set. Check its status in the Status column. Either libPrep_pending, libPrep_reserved, libPrep_done, planned, Voided, or Run are displayed.
  13. Notice a new icon for AmpliSeq™ on Ion Chef™ in the Lib Prep Type column.
  14. Monitor the Ion Chef™ library and templating steps from Monitor > Ion Chef. When the sequencing run is complete, view the Ion Chef™ run report.
  15. Click Data, Completed Runs & Reports, then select your Ion Chef™ run.
  16. Scroll to the bottom of the Run Summary screen, then select Chef Summary.
  17. Review the Chef Library Prep Info and Chef Template Prep Info sections.
Parent topic: Plan an instrument run