FreeBayes advanced settings
These parameters control the behavior of the FreeBayes module, which generates a list of variant candidates.
Again, these parameters are recommended for advanced users only.
Parameter |
Description |
---|---|
allow_indels |
Candidate generation parameter: Allow indel candidates to be generated when set Allowed values: 1 = generate indel candidates, 0 = don't generate Suggested trial value 1 |
allow_snps |
Candidate generation parameter: Allow SNP candidates to be generated when set Allowed values: 1 = generate SNP hypotheses, 0 = don't generate Suggested trial value 1 |
allow_mnps |
Candidate generation parameter: Allow MNP candidates to be generated when set Allowed values: 1 = generate MNP hypotheses, 0 = don't generate Suggested trial value 1 |
allow_complex |
Candidate generation parameter: Allow complex variant candidates to be generated when set Allowed values: 1 = generate MNP hypotheses, 0 = don't generate Suggested trial value 1 |
gen_min_alt_allele_freq |
A candidate generation parameter: A non-HP-indel candidate needs to have an allele frequency greater than this value in the pileup Allowed values: Decimal numbers between 0 and 1 Suggested trial value between 0.02 and 0.15 |
gen_min_indel_alt_allele_freq |
A candidate generation parameter: An HP-indel candidate needs to have an allele frequency greater than this value in the pileup Allowed values: Decimal numbers between 0 and 1 Suggested trial value between 0.02 and 0.15 |
gen_min_coverage |
A candidate generation parameter: A variant candidate location needs to have coverage depth greater than this value Allowed values: Integers >= 0 Suggested trial value 6 |
min_mapping_qv |
A candidate generation and variant evaluation parameter: Minimum mapping quality value required for a read to be considered (for both candidate generation and variant evaluation) Allowed values: Integers >= 0 Suggested trial value 4 |
read_snp_limit |
Do not use reads with number of snps above this Allowed values: Integers >= 0 Suggested trial value 10 |
read_max_mismatch_fraction |
A candidate generation parameter: Ignore reads with fraction of mismatch greater than this value Allowed values: Decimal numbers between 0 and 1 Suggested trial value 1.0 |
Parameter |
Comments |
---|---|
tvcargs |
This field is for internal use. |
tmapargs |
The desirable arguments for aligning the BAM file. Recommended value: "tmap mapall . . . -J 25 --end-repair 15 --do -repeat-clip --context stage1 map4" (ampliseq), "tmap" (others)| |
The Variant Caller parameter settings are saved in templates but are not saved in run plans. Parameter changes that you make in a run plan affect only that specific run.
When you change Variant Caller parameter settings in a template, your changes affect all users who create run plans from that template.