You can use Read Alignment to evaluate the quality of the sequencing reaction and the quality of the underlying library where an accurate reference is available. Reads are aligned to a reference genome. Any discrepancy in alignment to a reference (whether biological or technical, meaning a real variant or a sequencing error) is listed as a mismatch. Alignment performance metrics are reported depending on how many misaligned bases are allowed. Torrent Suite™ Software reports alignment performance at two quality levels:

• AQ20

• Perfect