Prerequisites

The following are prerequisites to uploading a new reference file:

Create a FASTA format reference sequence file (on your client machine).
Note:
FASTA files can be found at: http://www.ncbi.nlm.nih.gov/sites/genome download the FASTA file to your local client machine.

Note:
It is important that the format of your FASTA file conform to Ion Torrent™ requirements.

Note:
When working with larger genomes, performance improves if you first zip the FASTA file. The create index tool supports a zip archive, provided the file contains only a single FASTA file.
Prepare a descriptive name for the genome.
Prepare the short name for the genome.
Prepare a version for the genome.
Know the number of reads to randomly sample for alignment.
Prepare a regions of interest file or hotspots file (on your client machine).
Note:
To provide a better uploading experience, Adobe^® Flash^® or Microsoft™ Silverlight^® plugins are required to be installed for your browser. You may need to contact your local system administrator for assistance.
- Silverlight^® can be downloaded from http://www.silverlight.net/getstarted/.
- Adobe^® Flash^® can be downloaded from Flash^® player/.