Torrent Suite Software 5.4 Help

Individual barcode view

Click on any barcode of interest to see similar graphs of the barcode alone.
Reference table - Plot showing the number of genes with reads in log10 counting bins.

Gene Mapping Summary - Summary of reads mapped to genes of annotated reference.

Base Mapping Summary - Summary of base reads aligned to genetic features of an annotated reference.

Normalized Transcript Coverage - A plot of normalized transcript coverage; the frequency of base reads with respect to the length of individual transcripts they are aligned to in the 3' to 5' orientation.

Gene Isoform Expression - Box plots showing variation of isoforms expressed at FPKM ≥ 0.3 for each set of genes grouped by the number of anticipated (annotated) isoforms. Whiskers are defined by points within Q1-1.5xIQR to Q3+1.5xIQR. Only genes with 25 or less isoforms are represented in this plot. The data and a plot for all genes are available for download using the download reports links at the bottom of the screen.