Torrent Suite Software 5.4 Help

TMAP map4 Options

This page describes the parameters for the TMAP map4 module. The map4 module is based on the BWA fastmap routine and searches for the maximum exact matches between the reads and reference.

Note:

The map4 module is the current default for TMAP.

Options supported with the TMAP map4 module (all are optional):

Option

Type

Default

Description

--context

--

off

Modifies the gap penalty in homopolymers to achieve more accurate alignments

--do-repeat-clip

--

off

Clips repetitive sequence ends of aligned reads

--hit-frac

FLOAT

0.2

The fraction of seed positions that are under the maximum

--end-repair

INT

0

Rescues false negatives by selectively forcing alignment at the 3' end of the read. The recommended value is 15.

--J

INT

off 2147483647

Rescues false negatives by selectively forcing alignment at the 3' end of the read. The recommended value is 25.

--seed-step

INT

8

The number of bases to increase the seed for each seed increase iteration (-1 to disable)

--min-seed-length

INT

-1

The minimum seed length to accept hits (-1 to disable)

--max-seed-length

INT

48

The maximum seed length to accept hits

--max-seed-length-adj-coef (-1 to disable)

FLOAT

2.0

maximum seed length adjustment coefficient (-1 to disable)

--max-iwidth

INT

20

The maximum interval size to accept a hit

--max-repr

INT

3

The maximum representative hits for repetitive hits

--rand-repr

INT

false

Choose the representative hits randomly. Otherwise uniformly

--use-min

Boolean

false

When seed stepping, try seeding when at least the minimum seed length is present. Otherwise, use the maximum seed length.

--min-seq-length

INT

-1

The minimum sequence length to examine (-1 to disable)

--max-seq-length

INT

-1

The maximum sequence length to examine (-1 to disable)