JavaScript must be enabled in order to use this site.Please enable JavaScript in your browser and refresh the page. Target Regions File Formats Target regions BED files use 3-column, 4-column, 6-column, and 8-column formats. 3-column Target Regions BED File Format 4-column Target Regions BED File Format 6-column Target Regions BED File Format 8-column Target Regions BED File Format BED files generated by AmpliSeq.com custom designs Parent topic: Manage Target Regions Files and Hotspot Files