Torrent Suite Software 5.4 Help

Create a planned run for mixed samples with a template

To plan a run for DNA and Fusion sample pairs and several individual Fusion or DNA samples, start with a fusions template, then alter it to accommodate single samples on the same chip. The example that follows is a mixed sample set consisting of two sample pairs, one DNA-only sample and two Fusion-only samples.

  1. Copy the Ion AmpliSeq™ Colon Lung template.
  2. Enter the number of samples.
  3. Deselect "Same sample for DNA and Fusions" option.
  4. Renumber the samples.
  5. Change DNA/RNA selections to match samples.
  6. Select cancer types to match samples.
  7. Select appropriate Ion Reporter workflows.
  8. Enter Relation.
  9. Enter gender.
  10. Enter Analysis set IDs.
  11. Click Plan Run.