Torrent Suite Software 5.4 Help

Create a planned run with AmpliSeq™ DNA template

AmpliSeq™ DNA/Exome/RNA templates (also known as panels) can be downloaded from AmpliSeq.com. The necessary BED files for those templates are automatically installed with the templates. Also, you can edit the templates that are downloaded from AmpliSeq.com or clone those templates to meet your specific needs. The AmpliSeq™ DNA templates are used to create planned runs for various AmpliSeq™ panels, such as Ion AmpliSeq™ Exome and Ion AmpliSeq™ Inherited Disease Panel. You can select your Ion Reporter™ account, kits, plugins, and parameter settings.

Note:

To modify default parameters, see Configure and select a custom analysis parameter set.

  1. In the Plan tab, click Templates, then in the Favorites list, select AmpliSeq DNA.
  2. Select a template that matches your panel. For instance, if you are using an Ion AmpliSeq™ Exome Panel, select the AmpliSeq™ DNA template with the same name.

    The wizard launches and displays the Plan screen.

  3. Add samples, ensure that the default settings, enter a plan name, then click Plan Run.
  4. Run the plan on your sequencing system.